杜仲乙醇提取物对脑老化模型小鼠血中氧化应激指标的影响

被引：7

作者：

马山 ^{[1
]}

孙金杰 ^{[2
]}

王欣 ^{[3
]}

李淼 ^{[4
]}

杜丽娜 ^{[1
,4
]}

田景振 ^{[1
]}

机构：

[1] 山东中医药大学

[2] 空军总医院

[3] 滨州市沾化区人民医院

[4] 军事医学科学院放射与辐射医学研究所

来源：

国际药学研究杂志 | 2016年 / 43卷 / 05期

关键词：

抗氧化活性; 脑老化; 杜仲; 中药提取物;

D O I：

10.13220/j.cnki.jipr.2016.05.021

中图分类号：

R285.5 [中药实验药理]; R-332 [医用实验动物学];

学科分类号：

1008 ; 1001 ;

摘要：

目的探讨杜仲对脑老化模型小鼠血中氧化应激指标的影响。方法皮下注射D-半乳糖建立小鼠老龄化模型,杜仲乙醇提取物灌胃给药。采用流式细胞仪测定不同组小鼠脑细胞凋亡率,采用ELISA试剂盒测定一系列氧化指标,包括丙二醛(MDA)、超氧化物歧化酶(SOD)、蛋白质羰基含量(PCO)和一氧化氮(NO)含量。结果流式细胞仪测定结果显示,与模型组相比,杜仲提取物组小鼠脑细胞凋亡率明显下降(P<0.01)。ELISA测定结果表明,与正常组相比,杜仲组小鼠血中NO和SOD水平显著升高(P<0.01)。与模型组相比,杜仲组小鼠血中MDA和PCO水平显著降低(P<0.01),而SOD水平显著升高(P<0.01)。结论杜仲提取物可能通过有效减轻氧化损伤而发挥其抗脑老化作用。

引用

页码：922 / 925+930 +930

页数：5

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