大茴香酸-硫酸荧光体系测定黄芪甲苷

被引：7

作者：

刘养清

杜鸣

徐秉玖

机构：

[1] 山西师范大学化学系!山西临汾

[2] 北京医科大学药学院分析化学与药物分析研究室!北京

来源：

药学学报 | 2000年 / 07期

关键词：

黄芪甲苷; 大茴香酸; 黄芪; 中药复方补阳还五汤; 荧光分光光度法;

D O I：

10.16438/j.0513-4870.2000.07.017

中图分类号：

R284 [中药化学];

学科分类号：

1008 ;

摘要：

AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0 2 to 40 μg·mL -1 . The detection limit is 0 02 μg·mL -1 . The recoveries of samples are between 98 65% and 102 4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully.

引用

页码：544 / 546

页数：3

共 5 条

[1]

Effects on astragalus saponin 1, on cAMP and cGMP level in plasma and DNA synthesis in regenerating liver. Zhang YD,Shen JP,Wang YL,et al. Acta Pharmacological Sinica . 1984

[2]

Study on determination of astragaloside. Lu YX. Chin Tradit Pat Med (in Chinese) . 1996

[3]

Content determination of astragaloside in Astragalus membranaceus Bge. Yu JH,Zao ZZ,Zhang Q. Bull Chin Mater Med (in Chinese) . 1986

[4]

Effects on blood pressure and inflammation of astragalous saponin 1, a principle isolated from Astragalus membranaceus Bge. Zhang YD,Wang YL,Shen JP,et al. Acta Pharmacological Sinica . 1984

[5]

Determination of astragaloside content in Ligan Long by TLC. Yang BY,Yu YQ,Zhang XY,et al. J Chin Med Pharmacol (in Chinese) . 1995

← 1 →