姜黄素对APP/PS1双转基因小鼠海马组织中酵母自噬相关基因Beclin1表达和淀粉样蛋白生成的影响

被引：13

作者：

王晨 ^{[1
,2
,3
]}

滕志朋 ^{[1
,2
,3
]}

张雄 ^{[2
,3
]}

李昱 ^{[1
,2
,3
]}

机构：

[1] 重庆医科大学病理学教研室

[2] 重庆医科大学神经科学中心

[3] 重庆市神经生物学重点实验室

来源：

中国生物制品学杂志 | 2013年 / 26卷 / 11期

关键词：

姜黄素; 阿尔茨海默病; Beclin1; 淀粉样蛋白;

D O I：

10.13200/j.cnki.cjb.001947

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的观察姜黄素(curcumin)对APP/PS1(β-amyloid precursop protein/presenilin-1)双转基因阿尔茨海默病(alzheimer disease,AD)模型小鼠大脑海马组织中自噬相关基因Beclin1的表达以及淀粉样蛋白β(β-amyloid,Aβ)42生成的影响。方法将APP/PS1双转基因小鼠随机分为姜黄素高、低剂量模型组和空白对照组,每组10只,高、低剂量组分别将姜黄素按1 000和160 mg/kg加入饲料中喂养,连续给药6个月,空白对照组给予正常饲料,采用免疫组化和Western blot法检测姜黄素对小鼠大脑海马中Beclin1的表达和Aβ42生成的作用。结果与空白对照组相比,高、低剂量模型组小鼠大脑海马中Beclin1蛋白阳性细胞数及表达量均明显增多(P均<0.05),Beclin1蛋白的表达无剂量依赖性;Aβ42的生成随药物浓度的增加明显减少(P<0.05)。结论姜黄素可诱导APP/PS1双转基因小鼠大脑海马组织中Beclin1蛋白的表达,其机制可能是姜黄素通过促进自噬作用而抑制了Aβ42的生成,从而发挥其保护作用。

引用

页码：1625 / 1628

页数：4

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