miRNA在急性胰腺炎诊断和评估病情严重程度的作用

被引：9

作者：

傅冬阳

黄元林

陈建洪

机构：

[1] 南京军区福州总医院第一附属医院急诊科

来源：

临床和实验医学杂志 | 2016年 / 15卷 / 14期

关键词：

急性胰腺炎; microRNA; 诊断;

D O I：

暂无

中图分类号：

R576 [胰腺疾病];

学科分类号：

摘要：

目的探讨急性胰腺炎患者血清microRNA(miRNA)的表达差异及miRNA与急性胰腺炎严重程度的可能关系。方法收集急性轻度(A组)、中重度胰腺炎(B组)各30例的临床资料,健康人30例(C组)作为对照组。用miRNA表达谱芯片分析A组、B组及C组10例血清样本中的miRNA表达谱,并扩大样本(A、B、C组所有标本)应用实时荧光定量PCR方法进行验证;筛选出与急性胰腺炎相关的血清miRNA。比较A组和B组血清miRNA的表达差异。结果 24个miRNA在急性胰腺炎血清中的表达有明显变化,其中4个表达明显下调,20个miRNA表达明显上调。与对照组比较,急性胰腺炎患者miRNA-216a、miRNA-155、miRNA-196a和miRNA-375的表达有明显上调,miRNA-21表达下调。B组miRNA-375、miRNA-216a的表达明显高于A组,差异有统计学意义(P<0.05)。结论 miRNA为急性胰腺炎的诊断及早期病情评估提供了新的依据。

引用

页码：1398 / 1401

页数：4

共 9 条

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