免疫接种乙型肝炎病毒外膜小S蛋白产生的细胞免疫反应

被引：17

作者：

施理

刘树人

孔祥平

易学端

张宜俊

机构：

[1] 全军传染病中心

[2] 全军传染病中心广东广州

[3] 广东广州

来源：

中华医院感染学杂志 | 2003年 / 08期

关键词：

细胞毒T淋巴细胞; 辅助T淋巴细胞; 小鼠; 接种; HBV外膜小S蛋白;

D O I：

暂无

中图分类号：

R392.1 [免疫生物学];

学科分类号：

100102 ;

摘要：

目的　研究BALB/c(H 2 d)小鼠免疫接种乙型肝炎病毒 (HBV)外膜小S蛋白 (HBVsmallSenvelopeprotein,S HBsAg)产生的细胞免疫反应。方法　BALB/c(H 2 d)小鼠腹腔接种 0～ 5 μg的S HBsAg ;4周后分离脾淋巴细胞 ,体外分别用特异性多肽、S HBsAg刺激 ,分别用51Cr释放实验3 H掺入实验检测细胞毒T淋巴细胞 (cy totoxicTlymphocyte,CTL)及辅助T淋巴细胞 (helperTlymphocyte,HTL)增殖实验。结果　CTL反应的靶细胞自然释放率 ,自然释放 /最大释放 ,平均数±标准差为 (2 8 76± 13 4 5 ) % ;分别接种 0 ,0 6 5 ,1 2 5 ,2 5 ,5 μgS HB sAg的BALB/c(H 2 d)小鼠脾淋巴细胞CTL特异性释放率 (平均数±标准差 )分别为 (31 2 1± 9 2 3) % ,(4 2 36±19 32 ) % ,(91 2 1± 2 2 97) % ,(6 9 2 5± 2 4 l3) % ,(5 1 4 9± 2 1 6 6 ) % ,均高于作为对照的 0组小鼠脾淋巴细胞CTL释放率 ,差异有统计学意义 (P <0 0 5 ) ;HTL增殖实验 ,用S HBsAg刺激的 0 6 5～ 5 μg实验组BALB/c(H 2 d)小鼠脾淋巴细胞cpm值 (平均数±标准差 )分别为 3830 2 4± 14 96 12 ,2 736 19±12 38 0 6 ,4 10 0 37±172 3 74 ,12 4 6 18± 10 88 88,均高于未用S HBsAg刺激的对照组BALB/c(H 2 d)小鼠脾淋巴细胞cpm值 ,差异有统计学意义 (P

引用

页码：16 / 19

页数：4

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