转番茄正义抗坏血酸过氧化物酶基因提高烟草耐盐能力

被引：14

作者：

孙卫红

李风

束德峰

董新纯

杨秀梅

孟庆伟

机构：

[1] 山东农业大学生命科学学院,作物生物学国家重点实验室

来源：

中国农业科学 | 2009年 / 04期

关键词：

烟草; NaCl胁迫; LetAPX; 转基因植株; 耐盐性;

D O I：

暂无

中图分类号：

S572 [烟草（菸草）];

学科分类号：

0901 ;

摘要：

【目的】探讨叶绿体类囊体膜抗坏血酸过氧化物酶(tAPX)与耐盐性的关系。【方法】从番茄叶片中分离到类囊体膜抗坏血酸过氧化物酶基因(LetAPX),利用农杆菌介导的叶盘法转入到烟草中。Northern杂交分析了LetAPX在盐胁迫(NaCl)下的表达特征。以野生型(WT),转正义LetAPX烟草株系T2-2(+)和T2-6(+)为试材,测定了盐胁迫条件下APX酶活性,过氧化氢(H2O2)含量,种子发芽率,植株的鲜重、干重,光合速率及叶绿素荧光参数。【结果】Northern杂交显示LetAPX已转到烟草基因组中,且基因的表达受盐胁迫的诱导。盐胁迫下转基因烟草的种子发芽率,植株的鲜重与干重,APX酶活性和清除H2O2的能力都显著高于野生型,并且转基因烟草比野生型具有更高的净光合速率(Pn)和PSII最大光化学效率(Fv/Fm)。【结论】LetAPX的过量表达有助于提高烟草的耐盐能力。

引用

页码：1165 / 1171

页数：7

共 29 条

[1]

Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Lee,YP,Kim,SH,Bang,JW,Lee,HS,Kwak,SS,Kwon,SY. Plant Cell Reports . 2007

[2] Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis [J].

Lu, Zhenqiang ;

Liu, Dali ;

Liu, Shenkui .

PLANT CELL REPORTS, 2007, 26 (10) :1909-1917

[3] Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts [J].

Young-Pyo, Lee ;

Kim, Sun-Hyung ;

Bang, Jae-Wook ;

Lee, Haeng-Soon ;

Kwak, Sang-Soo ;

Kwon, Suk-Yoon .

PLANT CELL REPORTS, 2007, 26 (05) :591-598

[4]

The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism[J] . Christine H. Foyer,Barry Halliwell. &nbspPlanta . 1976 (1)

[5] 番茄种子吸湿—回干处理对盐胁迫伤害的缓解效应 [J].

贺军民 ;

佘小平 ;

张键 .

园艺学报, 2000, (02) :123-126

[6]

Enhanced photochemical light utilization and decreased chilling-induced photoinhibition of photosystem II in cotton overexpressing genes encoding chloroplast-targeted antioxidant enzymes. Dmytro Kornyeyev,Barry A. Logan,Paxton Payton,Randy D. Allen and A. Scott Holaday. Physiologia Plantarum . 2001

[7] 设施蔬菜土壤次生盐渍化发生机理的研究现状与展望 [J].

郭文忠 ;

刘声锋 ;

李丁仁 ;

赵顺山 .

土壤, 2004, (01) :25-29

[8]

Inhibition by calcium of senescence of detached cucumber cotyledons, effect on ethylene and hydroperoxide production. Ferguson IB,Watkins CB,Harman JE. Plant Physiology . 1983

[9]

Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Chen GX,Asada K. Plant and Cell Physiology . 1989

[10]

Photosynthesis and Seed Production under Water-Deficit Conditions in Transgenic Tobacco Plants That Overexpress an Arabidopsis Ascorbate Peroxidase Gene. Yen J,Wang J,Tissue D,Holaday AC,Allen R,Zhang H. Crop Science . 2003

← 1 2 3 →