一株产漆酶菌株的筛选鉴定和发酵条件的研究

被引:9
作者
刘敏
张明
机构
[1] 安徽农业大学生命科学学院
关键词
绿色木霉; 愈创木酚; 形态学特征; 酶活力;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
以愈创木酚为底物,采用平板筛选法筛选得到一株产漆酶菌株WS1-2,形态学特性表明该菌属于绿色木霉。对产酶条件的初步研究结果表明,WS1-2菌株的产酶高峰期出现在接种培养后的第4d。与蔗糖、乳糖、半乳糖和可溶性淀粉相比,以葡萄糖为碳源时,发酵上清液的漆酶活力明显要高,最大值达230U/L。以NH4Cl为氮源,最有利于WS1-2漆酶的产生,漆酶活力最高可达到234U/L。0.01mmol/L的愈创木酚和ABTS对WS1-2产漆酶有明显的诱导作用,3~5mg/L的Tween-80可以明显提高WS1-2的产酶水平。
引用
收藏
页码:40 / 43
页数:4
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