石杉碱甲改善新生大鼠缺氧缺血性脑损伤和智能缺陷的实验研究

被引：21

作者：

王来栓

周瑾

邵肖梅

唐希灿

机构：

[1] 复旦大学附属儿科医院内科,中国科学院上海生命学院药物研究所,复旦大学附属儿科医院内科,中国科学院上海生命学院药物研究所上海,上海

来源：

中华儿科杂志 | 2003年 / 01期

关键词：

倍半萜类; 胆碱酯酶抑制剂; 缺氧缺血,脑; 认知障碍; 大鼠;

D O I：

暂无

中图分类号：

R722.12 [新生儿窒息];

学科分类号：

100202 ;

摘要：

目的　探讨胆碱酯酶抑制剂———石杉碱甲对新生大鼠缺氧缺血性脑损伤 (hypoxia ischemiabraindamage ,HIBD)智能缺陷的改善和保护作用。方法　将HIBD模型鼠随机分为安慰剂组(n =11)、小剂量石杉碱甲组 ( 0 0 5mg/kg ,n =12 )、大剂量石杉碱甲组 ( 0 1mg/kg,n =12 )和对照组(n =10 ,为假手术组动物 )。以Morris水迷宫成绩判断大鼠学习、记忆功能变化 ;以结扎侧纹状体、皮层、海马损失面积和海马CA1区锥体细胞密度变化及乙酰胆碱酯酶组化染色判定脑组织损伤程度。结果　HIBD后 5周安慰剂组大鼠的学习、记忆功能受损严重 ,水迷宫实验潜伏期 (找到平台时间 )较对照组延长 ( 44s与 3 0s,P <0 0 5 ) ,平台撤除象限搜索时间缩短 ( 14s与 40s,P <0 0 1) ,脑组织损失面积最重 ;而与安慰剂组相比大剂量石杉碱甲治疗明显减轻大鼠的学习、记忆功能障碍 (潜伏期 :3 4s与 44s ,P <0 0 5 ;搜索时间 :3 5s与 14s,P <0 0 1) ,脑组织损失面积较轻 ,小剂量石杉碱甲治疗组大鼠学习、记忆功能改善不明显 (潜伏期 :45s与 44s,P >0 0 5 ;空间搜索时间 :17s与 14s,P >0 0 5 ) ,脑组织损失面积也较重 ;大鼠学习、记忆功能缺陷与海马CA1区锥体细胞密度呈显著相关 (r=0 777,P <0 0 1)。结论　胆碱酯酶抑制剂———石杉碱

引用

页码：46 / 49

页数：4

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