长春花黄化植原体(PY)株系的检测与鉴定

被引：22

作者：

蔡红

孔宝华

陈海如

机构：

[1] 云南农业大学云南省植物病理重点实验室,云南农业大学云南省植物病理重点实验室,云南农业大学云南省植物病理重点实验室昆明,昆明,昆明

来源：

微生物学报 | 2003年 / 01期

关键词：

长春花; 黄化; 植原体; 16SrRNA基因; 核糖体蛋白基因;

D O I：

10.13343/j.cnki.wsxb.2003.01.018

中图分类号：

S432.4 [侵（传）染性病害];

学科分类号：

090401 [植物病理学];

摘要：

A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.

引用

页码：116 / 119

页数：4