以寡聚组氨酸为配基的高效亲和色谱研究

被引：3

作者：

余晓

赵睿

方梅

熊少祥

苏天升

刘国诠

机构：

[1] 中国科学院化学研究所分子科学中心!北京

[2] 中国科学院化学研究所分子科学中心!北

来源：

高等学校化学学报 | 2000年 / 04期

基金：

中国科学院基金;

关键词：

IgG; 寡聚组氨酸; 高效亲和色谱; 解离常数; 固相肽合成;

D O I：

暂无

中图分类号：

O657.7 [色谱分析];

学科分类号：

摘要：

A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3 0, 4 5, 7 0 and 10 0. It showed the specific interaction at pH 4 5. The dissociation constants of IgG and oligohistine media(1H, 3H, 5H) were 3 037×10 -6 , 2 459×10 -6 and 1 905×10 -6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine residue in oligopeptide chains. [WT5HZ]

引用

页码：532 / 534

页数：3