微滴数字PCR定量检测乳制品中单核细胞增生李斯特氏菌

被引：8

作者：

程逸宇

冯秋实

吴海晶

黄梦静

刘新梅

机构：

[1] 南京市食品药品监督检验院

来源：

中国乳品工业 | 2019年 / 47卷 / 12期

基金：

国家重点研发计划;

关键词：

乳制品; 单核细胞增生李斯特氏菌; 微滴数字PCR; 定量检测;

D O I：

暂无

中图分类号：

TS252.7 [产品标准与检验];

学科分类号：

摘要：

选取单核细胞增生李斯特氏菌iap基因为靶基因,利用微滴数字PCR(ddPCR)技术对乳制品中单核细胞增生李斯特氏菌进行定量检测。实验结果表明,本方法能够对含菌量在5×10～3～5×10～6CFU/mL之间的巴氏乳样品进行准确定量检测,检测下限和定量检测下限分别为5×10～2CFU/mL和5×10～3CFU/mL,检测的准确性和灵敏性均优于qPCR检测。

引用

页码：37 / 40

页数：4

共 34 条

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