基于PI3K/AKT/mTOR通路、HIF-1α、ES观察新风胶囊对佐剂关节炎大鼠滑膜血管新生的影响

被引：21

作者：

张晓军 ^{[1
]}

刘健 ^{[2
]}

万磊 ^{[2
]}

黄传兵 ^{[2
]}

机构：

[1] 安徽中医药大学

[2] 安徽中医药大学第一附属医院

来源：

中国免疫学杂志 | 2017年 / 33卷 / 04期

关键词：

佐剂关节炎; 血管新生; PI3K/AKT/mTOR通路; 新风胶囊;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:观察新风胶囊(XFC)对佐剂关节炎(AA)大鼠滑膜PI3K/AKT/mTOR通路的影响。方法:将大鼠随机分成正常对照组、模型对照组、甲氨蝶呤组、雷公藤多苷组、XFC组。采用免疫组化法检测滑膜微血管密度(MVD)表达,酶联免疫吸附法检测白介素(IL)-6、IL-10、HIF-1α、VEGF-A表达,免疫印迹法检测PI3K、AKT1、p-AKT1、mTOR、ES蛋白在滑膜血管中表达。结果:模型对照组滑膜组织MVD计数升高,血清IL-6、VEGF、HIF-1α和滑膜PI3K、AKT1、p-AKT1、mTOR、ES表达显著升高,血清IL-10表达量降低;治疗后,XFC组MVD呈高计数量,HIF-1α、IL-6、VEGF-A表达降低,滑膜PI3K、p-AKT1、mTOR、ES蛋白表达降低,血清IL-10升高。结论:XFC通过调节PI3K/AKT/mTOR信号通路、HIF-1α、ES表达改善AA滑膜血管新生。

引用

页码：533 / 536+541 +541

页数：5

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