5-氟尿苷的微生物转化

被引：14

作者：

阮期平

周长林

窦洁

吴梧桐

机构：

[1] 中国药科大学生物制药学院

来源：

微生物学报 | 2002年 / 01期

关键词：

5-氟尿苷; 微生物转化; 分离纯化;

D O I：

10.13343/j.cnki.wsxb.2002.01.020

中图分类号：

O626 [杂环化合物];

学科分类号：

摘要：

Conditions for biotransformation and purification of FUR were investigated.The result showed that when the cell concentration of E.aerogenes was 10%(w/v),the temperature and pH were 7.8 and 60℃ respectively,59.7% UR was converted to FUR.It also demonstrated that the ideal procedure for the purification of FUR is silica gel column chromatography by two elution systems (S 1∶CHCl 3∶CH 3OH∶H 2O 61∶13∶1,and S 2;CH 3COOCH 2CH 3∶CH 3OH∶CH 3COOH∶H 2O 12∶3∶3∶2)with the purity and yield of 98.0% and 86.0% respectively.

引用

页码：117 / 120

页数：4