簇毛麦1V染色体SSR标记的筛选

被引:13
作者
刘守斌
唐朝晖
尤明山
李保云
宋建民
刘广田
机构
[1] 中国农业大学作物学院植物遗传育种系
基金
国家自然科学基金重点项目; 北京市自然科学基金;
关键词
簇毛麦; SSR; 染色体特异的SSR标记;
D O I
暂无
中图分类号
S512.103.3 [];
学科分类号
摘要
选用位于普通小麦 1A、1B、1D染色体上的 32对微卫星引物对普通小麦中国春、簇毛麦、6个中国春 簇毛麦二体附加系和 1个普通小麦 簇毛麦二体代换系进行SSR分析 ,发现引物Xgwm4 98在簇毛麦中扩增出 2条长分别为 110bp和190bp的片段 (即Xgwm4 98 110和Xgwm4 98 190 ) ,这两个片段仅在簇毛麦、中国春 簇毛麦 1Ha附加系中出现 ,其余 2Ha、4Ha、5Ha、6Ha、7Ha附加系、3V代换系和中国春中都缺少这两个片段。进一步研究表明 ,这两个片段与簇毛麦的居群无关。因此 ,Xgwm4 98 110和Xgwm4 98 190为簇毛麦 1V染色体所特有 ,可以用来快速跟踪导入普通小麦背景中的簇毛麦的1V染色体。
引用
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页码:138 / 142
页数:5
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