巴斯德毕赤酵母表达外源蛋白的研究进展

被引:27
作者
龙晶
杜立新
机构
[1] 中国农业科学院北京畜牧兽医研究所国家畜禽分子育种中心
关键词
毕赤酵母; 生物学特性; 蛋白表达;
D O I
暂无
中图分类号
Q78 [基因工程(遗传工程)];
学科分类号
071007 [遗传学];
摘要
毕赤酵母表达系统是近年来发展起来的一种高效表达外源基因的表达系统。综述了毕赤酵母表达系统的起源、生物学特性、融合蛋白的表达以及影响蛋白表达量的因素。
引用
收藏
页码:72 / 76
页数:5
相关论文
共 10 条
[1]
酵母蛋白分泌途径的研究进展 [J].
张伟 ;
刘志敏 ;
陈惠鹏 .
生物技术通讯, 2006, (01) :81-83
[2]
毕赤酵母表达系统 [J].
韩雪清 ;
刘湘涛 ;
尹双辉 .
微生物学杂志, 2003, (04) :35-40
[3]
Combined use of GAP and AOX1 promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris [J].
Wu, JM ;
Lin, JC ;
Chieng, LL ;
Lee, CK ;
Hsu, TA .
ENZYME AND MICROBIAL TECHNOLOGY, 2003, 33 (04) :453-459
[4]
Effects of substrate feed rates on heterologous protein expression by Pichia pastoris in DO-stat fed-batch fermentation [J].
Lee, CY ;
Nakano, A ;
Shiomi, N ;
Lee, EK ;
Katoh, S .
ENZYME AND MICROBIAL TECHNOLOGY, 2003, 33 (04) :358-365
[5]
Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from.[J].L. B. Trinh;J. N. Phue;Joseph Shiloach.Biotechnology and Bioengineering.2003, 4
[6]
Expression, purification, and characterization of equine lactoferrin in Pichia pastoris [J].
Paramasivam, M ;
Saravanan, K ;
Uma, K ;
Sharma, S ;
Singh, TP ;
Srinivasan, A .
PROTEIN EXPRESSION AND PURIFICATION, 2002, 26 (01) :28-34
[7]
Recombinant protein expression in Pichia pastoris [J].
Cregg, JM ;
Cereghino, JL ;
Shi, JY ;
Higgins, DR .
MOLECULAR BIOTECHNOLOGY, 2000, 16 (01) :23-52
[8]
The yeast expression system for recombinant glycosyltransferases [J].
Malissard, M ;
Zeng, S ;
Berger, EG .
GLYCOCONJUGATE JOURNAL, 1999, 16 (02) :125-139
[9]
Heterologous protein expression in the methylotrophic yeast Pichia pastoris [J].
Cereghino, JL ;
Cregg, JM .
FEMS MICROBIOLOGY REVIEWS, 2000, 24 (01) :45-66
[10]
High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris..Lisheng Peng; Xiaofen Zhong; Jingxing Ou; Suilan Zheng; Jian Liao; Lei Wang and Anlong Xu;.Journal of Biotechnology.2004, 02