空肠弯曲菌多重PCR快速检测方法建立的研究

被引：12

作者：

吴高林 ^{[1
]}

乔昕 ^{[2
]}

袁宝君 ^{[2
]}

机构：

[1] 南京医学大学公共卫生学院营养食品卫生系

[2] 江苏省疾病预防控制中心食品卫生科

来源：

现代预防医学 | 2007年 / 02期

关键词：

空肠弯曲菌; 多重PCR; 快速检测;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

100215 ;

摘要：

[目的]建立一种能够快速检测鉴别样品中的空肠弯曲菌的多重PCR方法。[方法]根据空肠弯曲菌hipO、mapA、23S等基因序列,利用Primer Premier 5.0和Oligo 6软件设计了多对特异性引物,经过优化筛选出最佳引物组合,再对引物的浓度、Mg2+浓度、退火温度等条件进行优化后,建立了从样品中快速检测鉴别空肠弯曲菌的多重PCR方法。[结果]实验结果显示本方法的特异性较强,灵敏度高,达到15 cfu/ml。[结论]多重PCR方法对于样品中空肠弯曲菌的检测实验周期短,灵敏度高,操作简便,适宜推广应用于各卫生单位日常对样品中空肠弯曲菌污染的快速检测。

引用

页码：207 / 209

页数：3

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