人胎盘间充质干细胞对小鼠肺纤维化的影响及机制

被引：3

作者：

吴树才 ^{[1
]}

杨永辉 ^{[1
]}

李辉 ^{[1
]}

郭素敏 ^{[1
]}

李秀武 ^{[2
]}

杜杰杰 ^{[2
]}

宋利超 ^{[2
]}

高莉 ^{[2
]}

机构：

[1] 河北省胸科医院

[2] 河北师范大学生命科学学院

来源：

山东医药 | 2016年 / 56卷 / 12期

关键词：

肺纤维化; 胎盘间充质干细胞; 博来霉素; 血管内皮生长因子; 内皮素1; 血管生成素2; 小鼠;

D O I：

暂无

中图分类号：

R563 [肺疾病];

学科分类号：

1002 ; 100201 ;

摘要：

目的观察人胎盘间充质干细胞对小鼠肺纤维化的影响,并探讨其作用机制。方法采用组织块贴壁法分离并体外培养人胎盘间充质干细胞。将30只C57BL/6小鼠随机均分为观察组、对照组,均给予气管内注入博来霉素8.5 mg/kg建立肺纤维化模型。造模成功后,观察组尾静脉输注体外培养的人胎盘间充质干细胞0.3 m L(细胞数为1.0×106个),对照组注射等量生理盐水,1次/d,连续注射3天;处死小鼠,取肺组织,检测肺组织羟脯氨酸含量,采用Western blotting法检测肺组织血管内皮生长因子(VEGF)、内皮素1(ET-1)和血管生成素2(Ang-2)蛋白。结果观察组肺组织羟脯氨酸含量为(5.76±0.13)μg/m L,对照组为(8.13±0.87)μg/m L,两组比较P<0.01。观察组肺组织VEGF的相对表达量为52.7±4.7、ET-1为68.1±5.4、Ang-2为59.6±2.8,均较对照组(100)降低(P均<0.05)。结论人胎盘间充质干细胞可抑制小鼠肺组织纤维化形成;降低肺组织VEGF、ET-1和Ang-2表达可能是其作用机制。

引用

页码：13 / 15

页数：3

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