急性脑创伤后迟发性神经元死亡的实验观察附视频

被引：15

作者：

杨新宇

杨树源

张建

宁雪亮

王丽蕙

胡震

机构：

[1] 天津医科大学总医院神经外科

来源：

中华创伤杂志 | 2001年 / 09期

关键词：

脑损伤; 细胞死亡,神经元; 凋亡;

D O I：

暂无

中图分类号：

R651.15 [];

学科分类号：

摘要：

目的　探讨急性脑创伤后迟发性神经元损伤的病理形式及过程。　方法　以大鼠脑创伤模型和体外培养海马神经元为研究对象。采用光镜、电镜方法对模型的损伤情况进行形态观察 ;以DNA -Ladder、TUNEL法对神经元DNA损伤情况进行观察。　结果　光镜下观察见神经元出现变性 ;电镜下观察见神经元坏死、凋亡 ,伤后 1d时最严重 ;DNALadder实验可见梯度电泳带 ,TUNEL法伤后 2h神经元即有染色 ,伤后 1d最明显 ,伤后 7d时仍高。体外实验神经元划伤后崩解、坏死 ,正常神经元在划伤神经元培养液中培养后出现凋亡。　结论　急性脑创伤后由于多种因素的作用 ,导致迟发性神经元死亡 ,主要表现为坏死和凋亡 ,凋亡持续时间较长

引用

页码：6 / 8

页数：3

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