浅水湖泊沉积物脱氢酶活性的测定及其生态学意义

被引:30
作者
黄代中 [1 ,2 ]
肖文娟 [1 ,2 ]
刘云兵 [1 ,2 ]
刘竟远 [1 ]
周易勇 [1 ]
机构
[1] 中国科学院水生生物研究所
[2] 中国科学院研究生院
关键词
沉积物; 脱氢酶; TTC; 微生物活性; 有机质; 湖泊;
D O I
暂无
中图分类号
P343.3 [湖泊、水库];
学科分类号
070403 [天体物理学];
摘要
以武汉市浅水湖泊(月湖)为实验对象确定了沉积物脱氢酶活性(DHA)测定的最佳条件:以经甲醛灭菌的沉积物为对照,以氯化三苯基四氮唑为电子受体(0.4%),沉积物用量为0.5g,pH值为7.5,培养时间为3h.据上述条件测定的DHA比未优化条件下的相应值高3-5倍,且表现出与沉积物有机质含量更为显著的正相关关系.武汉东湖3个子湖共23个采样点沉积物样品的测试结果进一步证实了上述方法的可行性,同时初步揭示了湖泊沉积物脱氢酶反映微生物活性与有机质状态的生态学意义.
引用
收藏
页码:345 / 350
页数:6
相关论文
共 21 条
[1]
TTC─脱氢酶测定方法的探讨[J] 朱南文,闵航,陈美慈,赵宇华 中国沼气 1996, 02
[2]
TTC-脱氢酶活性测定法的改进[J] 牛志卿,刘建荣,吴国庆 微生物学通报 1994, 01
[3]
Soil microbial biomass; dehydrogenase activity; bacterial community structure in response to long-term fertilizer management[J] Haiyan Chu;Xiangui Lin;Takeshi Fujii;Sho Morimoto;Kazuyuki Yagi;Junli Hu;Jiabao Zhang Soil Biology and Biochemistry 2007,
[4]
Toxicity interaction of metals (Ag; Cu; Hg; Zn) to urease and dehydrogenase activities in soils[J] Sophie Chaperon;Sébastien Sauvé Soil Biology and Biochemistry 2007,
[5]
Iron and phosphorus effects on the growth of Cryptomonas sp. (Cryptophyceae) and their availability in sediments from the Pearl River Estuary; China[J] Huan-Xin Weng;Ya-Chao Qin;Xiang-Wei Sun;Hailiang Dong;Xiang-Hua Chen Estuarine; Coastal and Shelf Science 2007,
[6]
Towards an Optimized Protocol for Measuring Global Dehydrogenase Activity in Storm-Water Sediments (10 pp)[J] Manuelle Neto;Aurélie Ohannessian;Cécile Delolme;Jean-Philippe Bedell Journal of Soils and Sediments 2007,
[7]
Microbiological degradation index of soils in a semiarid climate[J] Felipe Bastida;José Luis Moreno;Teresa Hernández;Carlos García Soil Biology and Biochemistry 2006,
[8]
Dehydrogenase and phosphomonoesterase activities in groundnut ( Arachis hypogaea L.) field after diazinon; imidacloprid and lindane treatments[J] Jitendra Singh;Dileep K. Singh Chemosphere 2005,
[9]
Soil enzyme activities suggest advantages of conservation tillage practices in sorghum cultivation under subtropical conditions[J] A. Roldán;J.R. Salinas-García;M.M. Alguacil;E. Díaz;F. Caravaca Geoderma 2005,
[10]
Progress of eutrophication and change of chironomid fauna in Lake Yamanakako; Japan[J] Kimio Hirabayashi;Kazuya Yoshizawa;Norihiko Yoshida;Futaba Kazama Limnology 2004,