正常与脑梗死后大鼠来源的骨髓单个核细胞治疗缺血性脑卒中的效果对比

被引：6

作者：

王建平

余列

蒋超

陈明

王梦涵

欧春影

王新新

周晨光

机构：

[1] 郑州,郑州大学第五附属医院神经内科,郑州大学脑血管病研究所

来源：

中华行为医学与脑科学杂志 | 2013年 / 22卷 / 02期

关键词：

缺血性脑卒中; 细胞移植; 骨髓单个核细胞; 血管新生; 细胞因子;

D O I：

暂无

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

目的对比正常与梗死后大鼠来源的骨髓单个核细胞(Nor-BMMNCs;Isch-BMMNCs)移植治疗大脑中动脉栓塞(MCAO)大鼠的疗效及对血管新生效应的影响。方法线栓法制作大鼠MCAO模型;梯度离心法分别提取Nor-BMMNCs及Isch-BMMNCs;用酶联免疫吸附法(ELISA)检测常氧及低氧条件下体外培养BMMNCs的上清液中VEGF、bFGF、Ang-1的表达水平;MCAO 1d后行股静脉1×107BMMNCs或PBS输注并行mNSS;细胞移植后14 d行TTC染色及免疫荧光检测。结果低氧条件下培养1 d后, Isch-BMMNCs组分泌促血管因子的量[VEGF:(114.2±10.6)pg/ml, bFGF:(133.6±19.7)pg/ml, Ang-1:(131.3±21.9) pg/ml]明显高于Nor-BMMNCs组[VEGF:(96.0±12.6)pg/ml, bFGF:(107.1±19.6)pg/ml, Ang-1:(103.0±17.0)pg/ml], 差异有统计学意义(P<0.05); mNSS评分显示, 在细胞移植后10d, Isch-BMMNCs组神经缺损症状明显改善, 与Nor-BMMNCs组及PBS组比较, 差异有统计学意义(P<0.05);细胞移植14 d后, Isch-BMMNCs组微血管数目及脑梗死体积[(372.5±40.1)支/mm2, (18.7±4.5)%]与Nor-BMMNCs组[(314.0±40.1)支/mm2, (26.5±7.2)%]及PBS组[(222.3±56.7)个/mm2, (34.5±6.3)%]比较, 差异有统计学意义(P<0.05)。结论 Isch-BMMNCs治疗缺血性脑卒中的价值明显高于Nor-BMMNCs, 可能与其分泌促血管因子能力增强有关。

引用

页码：103 / 106

页数：4

共 18 条

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