大鼠胎鼠中脑神经干细胞的分离培养

被引:2
作者
李学坤
郭安臣
左萍萍
机构
[1] 中国医学科学院、中国协和医科大学基础医学院药理学系
[2] 中国医学科学院、中国协和医科大学基础医学院药理学系 北京市
[3] 北京市
关键词
大鼠胎鼠中脑; 神经干细胞; 分离; 培养;
D O I
暂无
中图分类号
R329 [人体组织学];
学科分类号
100101 ;
摘要
目的探讨神经干细胞分离、培养及鉴定的方法 ,并了解其生物学特性。方法分离大鼠胎鼠腹侧中脑组织 ,加入丝裂原bFGF进行克隆密度细胞培养。用免疫细胞化学方法进行神经干细胞及其分化细胞的鉴定。结果培养的神经干细胞具备神经干细胞的基本特性 ,以对称和不对称分裂方式增殖形成神经球 ,并分化为神经元、神经胶质细胞、少突胶质细胞。结论成功建立了神经干细胞的分离培养方法。
引用
收藏
页码:14 / 16+69
页数:4
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