玉米BADH基因的克隆与序列分析

<正> Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants. Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme. The BADH gene of Maize was cloned by RT-PCR and RACE. The gene is 1 762 bp in length, including an open reading frame of 1 515 bp. Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor. Its deduced amino acid sequence contains the conserved domain sequence "VTLELGGKSP" of ALDH, and a tripeptide SKL at its C-terminal, a signal targetting to the microbodies. The phylogenetic tree of 20 BADHs was constructed , which corresponds to the classical botanical division of plant, the BADH of maize is closest to the one of Oryza Sativa,GenBank accession No. AY587278.