表皮细胞生长因子对小鼠非酒精性脂肪肝炎TNF-α、CHOP的影响

被引：1

作者：

麦炜健 ^{[1
]}

徐扬志 ^{[2
]}

赵丹 ^{[2
]}

余柑祥 ^{[2
]}

郭佳念 ^{[2
]}

甘静娣 ^{[2
]}

王芝蕾 ^{[2
]}

卢倩廷 ^{[2
]}

林嘉恩 ^{[2
]}

陈垦 ^{[1
]}

机构：

[1] 广东药科大学临床医学院

[2] 广州医科大学附属第二医院消化内科

来源：

广东药科大学学报 | 2018年 / 34卷 / 06期

关键词：

表皮生长因子; 非酒精性脂肪性肝炎; 炎症因子; 内质网应激;

D O I：

10.16809/j.cnki.2096-3653.2018091001

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的探讨表皮生长因子(EGF)对小鼠细胞非酒精性脂肪性肝炎(NASH)模型炎症因子及内质网应激的影响。方法用蛋氨酸与胆碱缺乏(MCD)的培养基诱导AML12小鼠正常肝细胞株建立NASH模型,分别设空白对照组、NASH造模组、EGF组,Western blot检测各组肿瘤坏死因子α(TNF-α)、C/EBP同源蛋白(CHOP)蛋白表达,油红O染色检测细胞内脂质沉积程度,实时定量荧光PCR(real-time PCR)检测TNF-α、CHOP的mRNA转录水平。结果 EGF组TNF-α、CHOP的蛋白及mRNA相对表达量与NASH组比较显著下降(P<0.05),脂质沉积变化不明显。结论 EGF能明显下调NASH中TNF-α、CHOP的表达水平,但对脂质沉积无明显改变。

引用

页码：724 / 727

页数：4

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