龙胆苦甙对减轻乳鼠心肌缺血再灌注损伤的细胞研究

被引：11

作者：

陈浩 ^{[1
]}

乔慧莲 ^{[2
]}

张磊 ^{[3
]}

肖丹 ^{[3
]}

陈赵云 ^{[3
]}

易定华 ^{[1
]}

机构：

[1] 第四军医大学西京医院心血管外科

[2] 第四军医大学人体解剖与组织胚胎学教研室

[3] 第四军医大学流行病学教研室

来源：

中华临床医师杂志(电子版) | 2013年 / 7卷 / 23期

关键词：

再灌注损伤; 龙胆苦甙; Akt/Gsk3β信号通路;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察龙胆苦甙(Gentiopicroside,GPS)后处理对离体心肌细胞缺血/再灌注损伤(ischemia and reperfusion injury,I/R)的拮抗作用及其可能的机制。方法采用SD大鼠乳鼠培养心肌原代细胞,用缺氧复氧模型模拟缺血再灌注(stimulated ischemia reperfusion,SI/R)。实验分为正常对照组(Control+ScrambleRNA+Veh);缺氧复氧组(I/R+ScrambleRNA+Veh);缺氧复氧+龙胆苦甙后处理组(I/R+ScrambleRNA+GPS组)以及缺氧复氧+AktSiRNA+龙胆苦甙后处理组(I/R+AktSiRNA+GPS)。采用化学法缺氧复氧模型,缺氧2 h,复氧4 h。复氧前给予GPS药物,检测心肌细胞乳酸脱氢酶(LDH)以及TUNEL染色确定心肌细胞的损伤程度以及抑制Akt表达后GPS的保护作用变化。结果与I/R+ScrambleRNA+Veh组相比,I/R+ScrambleRNA+GPS组LDH明显降低(P<0.01),TUNEL染色阳性率增加减少(P<0.01),Akt/Gsk3β信号通路磷酸化程度明显增加(P<0.01)。与I/R+ScrambleRNA+GPS组相比,SI/R+SiAktRNA+GPS组,LDH活性显著增加(P<0.01),TUNEI染色阳性率增加(P<0.01),Gsk3β磷酸化程度减弱(P<0.01)。结论 GPS后处理对I/R大鼠心肌具有保护作用,其作用机制与AKT/Gsk3β信号通路的活化有关。

引用

页码：10731 / 10735

页数：5

共 14 条

[1]

Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts [J].

Kesavan, Rushendhiran ;

Potunuru, Uma Rani ;

Nastasijevic, Branislav ;

Avaneesh, T. ;

Joksic, Gordana ;

Dixit, Madhulika .

PLOS ONE, 2013, 8 (04)

[2]

In Vitro Inhibition and Induction of Human Liver Cytochrome P450 Enzymes by Gentiopicroside: Potent Effect on CYP2A6 [J].

Deng, Yating ;

Wang, Lu ;

Yang, Yong ;

Sun, Wenji ;

Xie, Renming ;

Liu, Xueying ;

Wang, Qingwei .

DRUG METABOLISM AND PHARMACOKINETICS, 2013, 28 (04) :339-344

[3]

An in vitro perspective to cholinesterase inhibitory and antioxidant activity of five Gentiana species and Gentianella caucasea [J].

Senol, Fatma Sezer ;

Tuzun, Canan Yagci ;

Toker, Gulnur ;

Orhan, Ilkay Erdogan .

INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 2012, 63 (07) :802-812

[4]

Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts.[J]..Journal of Pharmaceutical and Biomedical Analysis.2012,

[5]

Isolation of gentiopicroside from Gentianae Radix and its pharmacokinetics on liver ischemia/reperfusion rats.[J].Wan-Ling Chang-Liao;Chao-Feng Chien;Lie-Chwen Lin;Tung-Hu Tsai.Journal of Ethnopharmacology.2011, 2

[6]

Inhibitory Effects of Secoiridoids from the Roots of Gentiana straminea on Stimulus-induced Superoxide Generation, Phosphorylation and Translocation of Cytosolic Compounds to Plasma Membrane in Human Neutrophils [J].

Wei, Shihu ;

Chen, Guang ;

He, Wenfei ;

Chi, Haidong ;

Abe, Hideki ;

Yamashita, Koichi ;

Yokoyama, Masanori ;

Kodama, Hiroyuki .

PHYTOTHERAPY RESEARCH, 2012, 26 (02) :168-173

[7]

Differential Roles of GSK-3β During Myocardial Ischemia and Ischemia/Reperfusion [J].

Zhai, Peiyong ;

Sciarretta, Sebastiano ;

Galeotti, Jonathan ;

Volpe, Massimo ;

Sadoshima, Junichi .

CIRCULATION RESEARCH, 2011, 109 (05) :502-U123

[8]

Anti-apoptotic activity of gentiopicroside in D-galactosamine/lipopolysaccharide-induced murine fulminant hepatic failure [J].

Lian, Li-Hua ;

Wu, Yan-Ling ;

Wan, Ying ;

Li, Xin ;

Xie, Wen-Xue ;

Nan, Ji-Xing .

CHEMICO-BIOLOGICAL INTERACTIONS, 2010, 188 (01) :127-133

[9]

Down-regulation of NR2B receptors partially contributes to analgesic effects of Gentiopicroside in persistent inflammatory pain [J].

Chen, Lei ;

Liu, Jin-cheng ;

Zhang, Xiao-nan ;

Guo, Yan-yan ;

Xu, Zhao-hui ;

Cao, Wei ;

Sun, Xiao-li ;

Sun, Wen-ji ;

Zhao, Ming-Gao .

NEUROPHARMACOLOGY, 2008, 54 (08) :1175-1181

[10]

Inhibitory Potencies of Several Iridoids on Cyclooxygenase-1; Cyclooxygnase-2 Enzymes Activities; Tumor Necrosis Factor-α and Nitric Oxide Production In Vitro.[J].Kyoung Sik Park;Bong Hyun Kim;Il-Moo Chang.Evidence-Based Complementary and Alternative Medicine.2007, 1

← 1 2 →