益智仁醇提物通过抑制iNOS-NO保护6-OHDA引起的PC12细胞损伤

被引：10

作者：

廖婉莹 ^{[1
]}

张在军 ^{[1
]}

王美薇 ^{[1
]}

洪思佳 ^{[2
]}

林志秀 ^{[2
]}

李铭源 ^{[1
]}

机构：

[1] 澳门大学中华医药研究院

[2] 香港中文大学中医学院

来源：

中药药理与临床 | 2010年 / 26卷 / 04期

关键词：

益智仁提取物; 神经保护; 帕金森病; PC12细胞; 一氧化氮; 诱导型一氧化氮合酶;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的:探讨益智仁乙醇提取物(Alpiniae oxyphyllae ethanolic extract,AOE)对6-羟基多巴胺(6-hydroxydopamine,6-OH-DA)在PC12细胞上引起的神经损伤的保护作用及其机制。方法:采用4-甲基偶氮唑蓝比色法(MTT法)和乳酸脱氢酶释放法(LDH法)检测PC12细胞的活力;DAF-FMdiacetate荧光染色检测细胞内一氧化氮(NO)的含量;采用免疫印迹法检测诱导型一氧化氮合酶(iNOS)的蛋白表达水平。结果:AOE能够剂量依赖性的保护PC12细胞的活力,减少6-OHDA引起的PC12细胞内LDH释放。作用机理研究证明AOE能够降低6-OHDA刺激的PC12细胞内NO含量增加。免疫印记杂交的结果显示AOE能够降低iNOS的表达。结论:AOE能保护6-OHDA诱导的PC12细胞损伤,其作用机制可能与降低NO的产生和iNOS的表达。该研究中证实的AOE神经保护效应对帕金森病的防治可能具有一定的作用。

引用

页码：31 / 35

页数：5

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