栝楼桂枝汤的血清药物化学研究

被引：30

作者：

许文 ^{[1
,2
]}

吴献 ^{[1
]}

黄鸣清 ^{[1
,2
]}

林羽 ^{[1
]}

徐伟 ^{[1
,2
]}

褚克丹 ^{[1
,2
]}

林娜 ^{[3
]}

陈立典 ^{[4
]}

机构：

[1] 福建中医药大学药学院

[2] 福建中医药大学生物医药研发中心

[3] 中国中医科学院中药研究所

[4] 福建中医药大学康复医学院

来源：

中草药 | 2017年 / 48卷 / 10期

关键词：

栝楼桂枝汤; 血清药物化学; 移行成分; 高效液相色谱-四极杆-飞行时间质谱; 单萜苷类; 黄酮类;

D O I：

暂无

中图分类号：

R284.1 [化学分析与鉴定];

学科分类号：

100804 [中药化学];

摘要：

目的对栝楼桂枝汤进行血清药物化学成分研究,阐明其体内药效物质基础。方法通过高效液相串联飞行时间质谱(HPLC-Q-TOF-MS)法快速鉴定栝楼桂枝汤经大鼠ig给药后吸收入血的化学成分。HPLC-Q-TOF-MS法采用Shimadzu Inertsil ODS-SP(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%甲酸水为流动相进行梯度洗脱,采用负离子模式扫描,对空白血清、大鼠ig栝楼桂枝汤后的含药血清以及栝楼桂枝汤提取原液进行对比分析,确定血中移行成分。结果通过比较色谱峰保留时间、质谱信息、质谱裂解方式,共确认了栝楼桂枝汤42个吸收入血成分,包括单萜苷类、黄酮类、酚酸类、姜酚类、三萜皂苷类及没食子酰糖类等。结论建立的HPLC-Q-TOF-MS鉴定栝楼桂枝汤血清药物化学成分的分析方法简便、快速、准确,为揭示栝楼桂枝汤对脑卒中后康复,在体内发挥神经保护作用的药效物质提供实验依据。

引用

页码：2033 / 2043

页数：11

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