三氧化二砷对人肝癌细胞的体外作用及其机制

被引：8

作者：

王兴武

孙菊杰

魏玲

宋现让

机构：

[1] 山东省肿瘤医院

来源：

中国医院药学杂志 | 2006年 / 12期

关键词：

三氧化二砷; 肝癌细胞株; bcl-2蛋白; 增殖细胞核抗原; 细胞周期; 流式细胞术;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

100214 ;

摘要：

目的:观察三氧化二砷(As2O3)对人肝癌细胞BEL7402的体外作用及其机制。方法:采用MTT法观察As2O3对BEL7402细胞的生长抑制率;流式细胞仪测定经不同浓度As2O3处理后的BEL7402细胞周期、凋亡相关蛋白bcl-2阳性细胞百分率、增殖细胞核抗原(PCNA)变化。结果:As2O3可显著抑制BEL7402细胞生长,且剂量-效应关系显著(r=0.965 0,P<0.01),半数抑制浓度(IC50)为4.93μmol.L-1;As2O3能显著下调PCNA蛋白表达(P<0.01),并使细胞周期阻滞在G2/M期,但对bcl-2表达无影响(P>0.05)。结论:As2O3可有效抑制人肝癌BEL7402细胞生长,其机制可能与下调PCNA表达及阻滞细胞周期有关。

引用

页码：1500 / 1504

页数：5

共 8 条

[1] AsO诱导人肝癌细胞凋亡及对Fas表达和钙含量的影响 [J].

魏玲 ;

王兴武 ;

宋现让 ;

左文述 .

中国药理学通报, 2005, (11) :1399-1400

[2]

Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide[J]. Zhong-Ying Shen Jian Shen Qiao-Shan Li Department of Pathology,Medical College of Shantou University,Shantou 515031,Guandong Province,China Cai-Yun Chen Central Lab.Medical College of Shantou University Jiong-Yu Chen Central Lab.of Tumor Hospital,Medical College of Shantou University Yi Zeng Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China.

[3] mCICR is required for As2O3-induced permeability transition pore opening and cytochrome c release from mitochondria [J].

Xuemei Tian ;

Xiaodong Ma ;

Dongfang Qiao ;

Ande Ma ;

Fang Yan ;

Xingxu Huang .

Molecular and Cellular Biochemistry, 2005, 277 :33-42

[4]

Acute promyelocytic leukemia: recent advances in therapy and molecular basis of response to arsenic therapies[J] . Wen-Chien Chou,Chi V Dang.Current Opinion in Hematology . 2005 (1)

[5] Arsenic trioxide induces G2/M growth arrest and apoptosis after caspase-3 activation and bcl-2 phosphorylation in promonocytic U937 cells [J].

Park, JW ;

Choi, YJ ;

Jang, MA ;

Baek, SH ;

Lim, JH ;

Passaniti, T ;

Kwon, TK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (04) :726-734

[6] Potential role of caspase-3 and-9 in arsenic trioxide-mediated apoptosis in PCI-1 head and neck cancer cells [J].

Seol, JG ;

Park, WH ;

Kim, ES ;

Jung, CW ;

Hyun, JM ;

Lee, YY ;

Kim, BK .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2001, 18 (02) :249-255

[7] Induction of apoptosis and inhibition of human gastric cancer MGC-803 cell growth by arsenic trioxide [J].

Zhang, TC ;

Cao, EH ;

Li, JF ;

Ma, W ;

Qin, JF .

EUROPEAN JOURNAL OF CANCER, 1999, 35 (08) :1258-1263

[8]

Inhibition of cell proliferation and the action mechanisms of arsenic trioxide (As2O on human breast cancer cells. Chow SK,Chan JY,Fung KP. Journal of Cellular Biochemistry . 2004

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