银杏内酯B对大鼠中性白细胞花生四烯酸代谢酶和细胞内钙水平的影响

被引：29

作者：

周龙恩

王文杰

白金叶

程桂芳

机构：

[1] 中国医学科学院、中国协和医科大学药物研究所!北京,中国医学科学院、中国协和医科大学药物研究所!北京,中国医学科学院、中国协和医科大学药物研究所!北京,中国医学科学院、中国协和医科大学药物研究所!北京

来源：

药学学报 | 2001年 / 02期

关键词：

银杏内酯B; 血小板活化因子; 磷脂酶A2; 5-脂氧酶; 细胞内游离钙;

D O I：

10.16438/j.0513-4870.2001.02.004

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

目的　观察银杏内酯B对大鼠中性白细胞花生四烯酸代谢酶及细胞内钙水平的影响。方法　反相高效液相色谱及Fura 2 /AM荧光指示剂法。结果　银杏内酯B在 0 1- 10 μmol·L-1范围内 ,使花生四烯酸释放量降低10 9% - 2 2 2 % ;0 1- 5 0 μmol·L-1时 ,LTB4 和 5 HETE生成量分别降低 2 9 4% - 88 6 %和 2 6 2 % - 89 3 % ;在0 1- 10 0 μmol·L-1使PAF和fMLP刺激引起的细胞内游离钙浓度分别降低 13 9% - 5 1 4%和 2 2 % - 36 6 %。结论　银杏内酯B能抑制体外大鼠中性白细胞花生四烯酸代谢酶的活性及细胞内游离钙浓度的升高

引用

页码：92 / 95

页数：4

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