急性心肌梗死大鼠血清血管内皮生长因子含量的变化

被引：14

作者：

尹瑞兴

冯建章

姚震

机构：

[1] 广西医科大学附一院心血管病研究所!广西南宁,广东省心血管病研究所心内科!广东广州,广东省心血管病研究所心内科!广东广州

来源：

中国病理生理杂志 | 2000年 / 01期

关键词：

心肌梗塞; 内皮; 生长物质; 酶连接免疫吸附测定;

D O I：

暂无

中图分类号：

R542.22 [];

学科分类号：

1002 ; 100201 ;

摘要：

目的和方法 :探讨急性心肌梗死大鼠模型血清血管内皮生长因子 (VEGF)含量的变化及其意义。 88只雄性SD大鼠 ,其中 80只结扎左冠状动脉导致心肌梗死 ,8只开胸但不结扎冠状动脉作对照组。于心肌梗死后 1h ,3h ,6h ,12h ,2 4h和 2d ,3d ,5d ,7d ,14d分别从右心房抽取血样品 (n =8)。用敏感、特异的酶连接免疫吸附测定法测定血清样品中的VEGF含量。结果 :8只假手术对照鼠的血清VEGF浓度为 (6 6 99± 17 83)pg/mL。心肌梗死后 6h组血清VEGF水平达到 (12 5 6 8± 2 8 0 7)pg/mL(与对照组比P <0 0 1) ,结扎后 2 4h组达高峰 (2 40 6 1± 70 6 3pg/mL ,与对照组比P <0 0 1) ,然后逐渐下降 ,但术后 14d组仍显著高于对照组 (10 7 6 4±30 13pg/mL ,P <0 0 1)。结论 :心肌梗死大鼠血清VEGF水平持续显著升高 ,可能对心肌梗死后的血管生成起重要作用

引用

页码：29 / 31

页数：3

共 10 条

[1]

Regulation of vascular endothelial growth factor in cardiac myocytes. Levy AP,Levy NS,Loscalzo J,et al. Circulation Research . 1995

[2]

Enhanced microvascular relaxations to VEGF and bFGF in chronically ischemic porcine myocardium. Sellke FW,Wang SY,Stamler A,et al. American Journal of Physiology . 1996

[3]

Angiogenic-induced enhancement of collateral blood flow to ischemic myocardium by vascular endothelial growth factor in dogs. Banai S,Jaklitsch MT,Shou M,et al. Circulation . 1994

[4]

Clinical evidence of angiogenesis following arterial gene transfer of phVEGF165 in patients with ischaemic limb. Isner JM,Pieczek A,Schainfeld R,et al. The Lancet . 1996

[5]

VEGF, flk-1, and flt-1 expression in a rat myocardial infarction model of angiogenesis. Li J,Brown LF,Hibberd MG,et al. American Journal of Physiology . 1996

[6]

Rapid induction of vascular endothelial growth factor expresstion by transient ischemia in rat heart. Hashimoto E,Ogita T,Nakaoka T,et al. American Journal of Physiology . 1994

[7]

Upregulation of vascular endothelial growth factor expression induced by myocardial ischaemia: implications for coronary angiogenesis. Banai S,Shweiki D,Pinson A,et al. Cardiovascular Research . 1994

[8]

Hypoxia is a strong inducer of vascular endothelial growth factor mRNA expression in the heart. Ladoux A,Frelin C. Biochemical and Biophysical Research Communications . 1993

[9]

Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages, and tumors. Berse B,Brown LF,Van de Water L,et al. Molecular Biology of the Cell . 1992

[10]

Stretch- induced VEGF expression in the heart. Li J,Hampton T,Morgan JP,et al. The Journal of Clinical Investigation . 1997

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