补中益气汤对A549/DDP肺腺癌耐药动物模型中凋亡相关信号分子caspase-9的影响

被引:10
作者
井欢
于丹
唐莹
刘春英
机构
[1] 辽宁中医药大学基础医学院病理生理教研室
关键词
补中益气汤; Caspase-9; 肺腺癌A549/DDP; BALB/c小鼠;
D O I
10.16695/j.cnki.1006-2947.2014.02.021
中图分类号
R734.2 [肺肿瘤];
学科分类号
100117 [系统生物医学];
摘要
目的研究补中益气汤对肺腺癌荷瘤BALB/c小鼠实体瘤中凋亡相关信号分子Caspase-9表达的影响,探讨其对肺腺癌可能的作用机制。方法体外培养肺腺癌A549/DDP细胞,接种健康BALB/c小鼠,荷瘤成功后予补中益气汤治疗,实验分为:荷瘤对照组,补中益气汤低剂量组及补中益气汤高剂量组。观察转移病灶情况,利用RT-PCR检测Caspase-9 mRNA的表达,免疫组化法检测Caspase-9蛋白表达。结果同荷瘤对照组相比,高、低剂量的补中益气汤均可上调凋亡相关信号分子Caspase-9 mRNA和蛋白的表达水平,并且补中益气汤高剂量组效果优于低剂量组。结论补中益气汤对肺腺癌的抑制作用可能与Caspase-9表达上调相关。
引用
收藏
页码:134 / 136+141 +141
页数:4
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