用微卫星序列构建羊草遗传指纹图谱

The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis （Trin.） Kitag) were constructed by Southern blot analysis of Ase Ⅰ, Dra Ⅰ, Eco RⅠ, Hin dⅢ, Nco Ⅰ, Mob Ⅰ, Rsa Ⅰ, Pst Ⅰ, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,（CT） 8,（GCTA） 4,（GGAT） 4 or （GACA） 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with （GCTA） 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and （CT） 8,（GCTA） 4,（GGAT） 4 or （GACA） 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when （GGAT） 4 was used, indicating satisfied results were obtained.