聚乙二醇修饰牛血清白蛋白的反应与分析

被引:5
作者
李伟军
郑春辉
张达磊
张颖
苏志国
机构
[1] 中国科学院化工冶金研究所生化工程国家重点实验室
[2] 中国科学院化工冶金研究所生化工程国家重点实验室 北京
[3] 北京
关键词
牛血清白蛋白; 聚乙二醇; 修饰; 分析; 毛细管电泳;
D O I
暂无
中图分类号
Q591.2 [蛋白质代谢];
学科分类号
071010 ; 081704 ;
摘要
采用N ,N′ 羰基二咪唑活化法活化单甲氧基聚乙二醇 5 0 0 0分子一端的羟基 ,对活化后的单甲氧基聚乙二醇分子进行了元素分析。用该活化产物对牛血清白蛋白的赖氨酸侧链氨基进行化学修饰。应用毛细管电泳对聚乙二醇修饰后的产物进行了分析 ,并与高效液相色谱分析结果作了对照研究 ,表明毛细管电泳对修饰后的牛血清白蛋白有更好的分析效果
引用
收藏
页码:164 / 166
页数:3
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