表皮葡萄球菌生物膜形成与ica操纵子的相关性研究

被引：15

作者：

王勇翔

李华林

李平洋

倪语星

闻玉梅

机构：

[1] 复旦大学上海医学院医学分子病毒学实验室

[2] 上海瑞金医院检验科

[3] 复旦大学上海医学院医学分子病毒学实验室上海

[4] 上海

来源：

中华微生物学和免疫学杂志 | 2003年 / 06期

关键词：

表皮葡萄球菌; 细菌生物膜; 半定量粘附实验; ica操纵子; 多聚酶链反应;

D O I：

暂无

中图分类号：

R346 [];

学科分类号：

1001 ;

摘要：

目的　研究表皮葡萄球菌 (表葡 )生物膜形成与ica操纵子存在之间的相关性并比较 3种检测表皮葡萄球菌生物膜形成的方法。方法　通过PCR法扩增icaA基因以检测ica操纵子的存在。用半定量粘附实验、扫描电子显微镜法和光学显微镜法检测自临床分离到的 19株表葡的粘附能力。结果　在 19株表葡中 ,通过PCR法测得 7株为icaA阳性 ,通过半定量粘附实验测得 5株icaA(+)菌株为粘附株 ;大多数菌株在含糖培养基中所测得的吸光度 (A)值大于不含糖培养基中所测得的A值。结论　ica操纵子的存在与表葡生物膜形成在统计学上显著相关 (P <0 .0 2 5 )。培养基中补充葡萄糖有利于大多数表葡的粘附。半定量粘附实验和光镜检测法适用于细菌粘附的初步测定和筛选 ,而扫描电镜检测法有更高的灵敏度以区分粘附菌株和非粘附菌株。

引用

页码：25 / 28

页数：4

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