二烯丙基硫化物对百草枯中毒急性肺损伤大鼠的抗氧化作用

被引：8

作者：

曹玉芳 ^{[1
]}

王洪武 ^{[1
]}

苏醒 ^{[1
]}

侯宇 ^{[1
]}

管军 ^{[1
]}

谢斌 ^{[1
]}

余燕梅 ^{[1
]}

王莲藕 ^{[1
]}

陈颖斌 ^{[1
]}

韩艳丽 ^{[1
]}

邱平 ^{[2
]}

宋维 ^{[3
]}

机构：

[1] 海口市人民医院重症医学科

[2] 海口市人民医院肾内科

[3] 海南省人民医院急诊科

来源：

中国药理学与毒理学杂志 | 2016年 / 30卷 / 05期

基金：

海南省自然科学基金;

关键词：

百草枯; 一氧化氮; 诱导型一氧化氮合酶; 急性肺损伤; 二烯丙基硫化物;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的探讨二烯丙基硫化物（DAS）对百草枯（PQ）中毒大鼠急性肺损伤的影响。方法 100只雄性Wistar大鼠随机分为5组,正常对照组、PQ 70 mg·kg-1模型组、DAS 25,50和100 mg·kg-1治疗组,每组20只。一次性ig给予PQ 70 mg·kg-1制备PQ中毒模型,DAS组于造模前、后30 min ip给予相应剂量药物,正常对照组ip给予等量生理盐水。各组分别于造模后1,3,6和12 h麻醉大鼠,取右肺下叶进行HE染色,光镜下观察肺组织病理损伤程度并评分;右肺上叶进行肺组织一氧化氮（NO）含量检测;左肺进行支气管肺泡灌洗获取肺泡巨噬细胞（AM）,培养24 h取上清,分光光度法测定诱导型一氧化氮合酶（i NOS）含量;培养至72 h,采用细胞免疫化学方法检测AM中i NOS蛋白的表达。结果与正常对照组比较,模型组肺泡结构破坏严重,病理评分明显升高（P<0.01）,肺组织中NO含量明显升高（P<0.01）,AM培养上清液中i NOS含量明显升高（P<0.01）,i NOS蛋白表达增加（P<0.05）;与模型组比较,DAS 50 mg·kg-1组在造模后3,6和12 h以及DAS 100 mg·kg-1组在造模后1,3,6和12 h肺泡结构损伤显著减轻,病理评分降低（P<0.05）;DAS25和50 mg·kg-1组肺组织中NO和i NOS含量及i NOS蛋白表达减少（P<0.05）,DAS 100 mg·kg-1组NO含量减少更为明显（P<0.01）。结论 DAS可能通过抑制PQ中毒大鼠肺组织中的氧化损伤因子减轻肺损伤。

引用

页码：526 / 531

页数：6

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