人参、藜芦合用对大鼠肝P450酶活性及mRNA表达的调控作用

被引：61

作者：

王宇光

高月

柴彪新

陈鹏

谭洪玲

赵永红

肖成荣

孙圆媛

朱力军

机构：

[1] 军事医学科学院放射医学研究所药理毒理研究室

来源：

中国中药杂志 | 2004年 / 04期

关键词：

十八反; 细胞色素P450; 逆转录聚合酶链式反应; 药物相互作用;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

摘要：

目的 :研究中药十八反中人参与藜芦合用对P4 5 0同工酶在酶活性及mRNA水平的调控作用。方法 :采用紫外分光光度法测定大鼠肝微粒体细胞色素P4 5 0与细胞色素b5含量及氨基比林N 脱甲基酶 (APND)、对硝基苯酚羟化酶 (pNPH)活性。采用RT PCR技术检测大鼠肝中 5种P4 5 0同工酶CYP1A1,CYP2B1/ 2 ,CYP2C11,CYP2E1及CYP3A1mRNA的表达。结果 :人参与藜芦合用可明显降低P4 5 0蛋白含量 ,可降低细胞色素b5的含量 ,单药及其与藜芦配伍都能使APND活性下降。在mRNA水平上 ,藜芦可诱导CYP2C11的基因表达 ,与人参合用后却使得CYP2C11的表达下降。藜芦与人参配伍诱导了CYP1A1的表达。人参可抑制CYP2B1/ 2的基因表达 ,与藜芦合用后却明显诱导了CYP2B1/ 2的表达 ,人参与藜芦合用后也明显诱导了CYP3A1的表达。结论 :人参与藜芦配伍前后对一些CYP亚型调控作用发生明显变化 ,可能存在基于药物代谢酶机理的相反作用 ,需进一步结合代谢研究加以综合分析 ,阐明相反作用的机理

引用

页码：82 / 86

页数：5

共 12 条

[1]

The induction of liver microsome cytochrome P450 by Glycyrrhiza uralensis and glycyrrhetinic acid in mice. Wen Y H,Ya W L,Yan N H,et al. Biomedical and Environmental Sciences . 1999

[2]

Modulation of cyrochrome P450dependent monooxygenases, glutathione and glutathione strangferase in rat liver by genposide from gardenia jasminoldes. Kang J J,Wang H W,Liu T Y,et al. Food and Chemical Toxicology . 1997

[3]

Human cytochromes P450. Julia A H,Ronald Estabrook,Michael Murray,et al. Molecular Aspects of Medicine . 1999

[4]

The Ah locus: genetic differences in toxicity, cancer.mutation and birth defects. Nebert D W. Critical Reviews in Toxicology . 1989

[5]

Comparison between Chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effect on rat liver CYP-catalysed drug metabolism. Guerra M C,Speroni E,Broccoli M,et al. Life Sciences . 2000

[6]

Brain cytochrome P450 are responsive to phenbarbital and tricyclic amines. Strobel H W,Cattaneo E,Adesnik M,et al. Pharmacological Research . 1989

[7]

Analysis of rat cytochrome P450 isoenzyeme expression using semi-quantitative transcriptase polymerase chain reaction (RT-PCR). Morris D L,Davila J C. Biochemical Pharmacology . 1996

[8]

p-Nitrophenol hydroxylation a microsomal oxidation which is highly inducible by ethanol. Lester A,Michele J. Drug Metabolism and Disposition . 1985

[9]

Effect of licorice and glycyrrhizin on liver CYP-dependent monooxygenases. Paolini M,Pozzetti L,Sapone A,et al. Life Sciences . 1998

[10]

The carbon monoxide-binding pigment of liver microsomes. Omura T,Sato R. Journal of Biological Chemistry . 1964

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