中国西北内陆干旱区大叶白麻野生居群遗传多样性研究(英文)

被引:17
作者
赵金凤 [1 ]
张卫明 [1 ,2 ]
彭雪梅 [1 ]
唐泽紫 [1 ]
顾龚平 [2 ]
陆长梅 [1 ]
机构
[1] 南京师范大学生命科学学院
[2] 南京野生植物综合利用研究院
关键词
Poacynum hendersonii; Wild population; Genetic diversity; RAPD;
D O I
10.16175/j.cnki.1009-4229.2008.04.015
中图分类号
S563.3 [大麻(白麻、线麻)];
学科分类号
0901 ;
摘要
[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.
引用
收藏
页码:39 / 43
页数:5
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