灵芝β-葡聚糖的碱提取及其抗肿瘤免疫调节研究

被引：38

作者：

张锦锦 ^{[1
,2
]}

陈菲菲 ^{[1
,2
]}

严展鹏 ^{[1
,2
]}

李畅 ^{[1
,2
]}

孙娥 ^{[1
,2
]}

罗毅 ^{[1
]}

谭晓斌 ^{[1
,2
]}

机构：

[1] 南京中医药大学附属中西医结合医院

[2] 江苏省中医药研究院国家中医药管理局中药释药系统重点研究室

来源：

药学学报 | 2020年 / 03期

关键词：

灵芝; β-葡聚糖; 响应面; 肿瘤免疫; 免疫调节;

D O I：

暂无

中图分类号：

R284 [中药化学]; R285 [中药药理学];

学科分类号：

100804 [中药化学]; 100806 [中药药理学];

摘要：

采用Box-Benhnken中心组合试验设计原理和响应面分析法,得到碱法提取灵芝颗粒性β-葡聚糖（Ganoderma lucidum particulateβ-glucan, GLG）最佳条件:提取温度55℃、时间8 h和料液比1∶20,提取得率为8.57%。本研究中动物实验获得江苏省中医药研究院伦理委员会的批准。体外免疫活性研究表明, GLG能促进巨噬细胞的激活并分泌促炎细胞因子,不仅能提高巨噬细胞的吞噬能力,还能促进巨噬细胞吞噬GLG自身,持续发挥免疫刺激作用; GLG还能促进小鼠脾淋巴细胞的增殖;此外,口服GLG能够显著抑制CT26荷瘤小鼠肿瘤的增殖,提高其脾脏中产生IFN-γ的CD4+T （Th1）细胞与CD8+T （CTL）细胞的比例,表明GLG能够促进具有杀伤肿瘤细胞作用的Th1和CTL的增殖与分化,从而增强机体抗肿瘤免疫。

引用

页码：512 / 521

页数：10

共 14 条

[1]

Curdlan oligosaccharides having higher immunostimulatory activity than curdlan in mice treated with cyclophosphamide[J] Jiqing Tang;Hongmin Zhen;Nannan Wang;Qiaojuan Yan;Hao Jing;Zhengqiang Jiang Carbohydrate Polymers 2018,

[2]

Effects and mechanisms of ultrasound- and alkali-assisted enzymolysis on production of water-soluble yeast β-glucan[J] Zhaomin Zheng;Qilin Huang;Xiaogang Luo;Yidong Xiao;Wenfei Cai;Huiyu Ma Bioresource Technology 2018,

[3]

Consumption of β-glucans to spice up T cell treatment of tumors: a review[J] de Graaff;Govers;Wichers;Debets Expert Opinion on Biological Therapy 2018,

[4]

Production of low-molecular weight soluble yeast β-glucan by an acid degradation method[J] Yuina Ishimoto;Ken-ichi Ishibashi;Daisuke Yamanaka;Yoshiyuki Adachi;Ken Kanzaki;Yoichiro Iwakura;Naohito Ohno International Journal of Biological Macromolecules 2018,

[5]

Vaccines targeting helper T cells for cancer immunotherapy[J] Marit Melssen;Craig L Slingluff Current Opinion in Immunology 2017,

[6]

Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice[J] Chengcheng Wang;Liang Feng;Jiayan Su;Li Cui;Dan Liu;Jun Yan;Chuanlin Ding;Xiaobin Tan;Xiaobin Jia Journal of Ethnopharmacology 2017,

[7]

An evaluation study of different methods for the production of β ‐D‐glucan from yeast biomass[J] Vassileios Varelas;Maria Liouni;Antony C. Calokerinos;Elias T. Nerantzis Drug Test. Analysis 2016,

[8]

Microparticulate β-glucan vaccine conjugates phagocytized by dendritic cells activate both naïve CD4 and CD8 T cells in vitro[J] Vanessa K. Berner;Sally A. duPre;Doug Redelman;Kenneth W. Hunter Cellular Immunology 2015,

[9]

d -Glucans from edible mushrooms: A review on the extraction; purification and chemical characterization approaches[J] Andrea Caroline Ruthes;Fhernanda Ribeiro Smiderle;Marcello Iacomini Carbohydrate Polymers 2015,

[10]

Immune modulation of the tumor microenvironment for enhancing cancer immunotherapy[J] Christel Devaud;Liza B John;Jennifer A Westwood;Phillip K Darcy;Michael H Kershaw OncoImmunology 2013,

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