E.tenella吉林株单卵囊的分离及PCR鉴定(英文)

被引：12

作者：

杨桂连 ^{[1
,2
]}

李建华 ^{[1
]}

张西臣 ^{[1
]}

赵权 ^{[1
,2
]}

宫鹏涛 ^{[1
]}

蔡亚南 ^{[1
]}

张国才 ^{[1
]}

机构：

[1] 吉林大学畜牧兽医学院

[2] 吉林农业大学动物科学技术学院

来源：

Agricultural Science & Technology | 2009年 / 10卷 / 03期

关键词：

E.tenella; Single-oocyst; Isolation; PCR identification;

D O I：

10.16175/j.cnki.1009-4229.2009.03.031

中图分类号：

S852.61 [病原细菌];

学科分类号：

摘要：

[Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. [Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenella. [Conclusion] The inoculation of single oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty, increased infection rate and provided good materials for biological research of coccidian.

引用

页码：55 / 57+62 +62

页数：4