聚合酶链反应检测患儿尿标本中的巨细胞病毒DNA

被引：6

作者：

孟潞英

张文炳

彭华国

董自正

石成华

常汝虚

刘琦

机构：

[1] 第一军医大学微生物学教研室,第一军医大学微生物学教研室,第一军医大学微生物学教研室,军事医学科学院生物工程研究所,军事医学科学院生物工程研究所,广州市儿童医院病毒室,广州市儿童医院病毒室

来源：

第一军医大学学报 | 1991年 / 04期

基金：

广东省自然科学基金;

关键词：

巨细胞病毒(HCMV); 聚合酶链反应(PCR); 尿; DNA;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

本文建立了聚合酶链反应(PCR)检测人巨细胞病毒(HCMV)方法,探讨了该方法的某些实验条件。用PCR检测24例临床患儿尿标本。证明PCR方法特异、敏感、简便而快速。同科不同属病毒如HSV、EBV等以及人源正常细胞均无假阳性结果:最小检出量可达0.1fg DNA,相当于6个基因拷贝;用水浴箱手控时间即可进行PCR循环,30次循环仅需97min30s;PCR和DNA杂交检测尿标本中HCMV阳性例数分别为20和14例。该结果证明HCMV感染儿童的广泛性和严重性,初步表明儿童肾病综合症与HCMV有关。

引用

页码：323 / 328

页数：6

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