DNA序列分析与PCR-SSCP分析结核分支杆菌利福平敏感性的比较

被引：5

作者：

黄海荣

何秀云

张宗德

张小刚

庄玉辉

马玙

机构：

[1] 北京市结核病胸部肿瘤研究所

[2] 解放军第医院

来源：

中国防痨杂志 | 2003年 / 04期

关键词：

DNA序列分析; 聚合酶链反应-单链构象多态性; 药物耐受性; 分支杆菌,结核;

D O I：

暂无

中图分类号：

R446.5 [微生物学检验];

学科分类号：

摘要：

目的　了解DNA序列分析与PCR -SSCP法分析利福平敏感性的价值。方法　应用DNA序列分析与PCR -SSCP法检测利福平敏感的结核分支杆菌临床分离株 2 5株、耐药株 4 1株的利福平耐药相关基因rpoB基因核心区域的突变情况。结果　 2 5株利福平敏感株DNA序列分析未检测到rpoB基因突变 ,4 1株耐利福平株中 38株发生突变 ,突变率为 92 .7% (38/ 4 1) ;2 5株利福平敏感的结核分支杆菌菌株PCR -SSCP带型与对照H37Rv株相同 ,4 1株耐利福平结核分支杆菌菌株中38株SSCP带型不同于对照株 ,提示有突变存在。与DNA序列分析相比 ,PCR -SSCP检测准确率为 93.9% (6 2 / 6 6 ) ,敏感度为 92 .1% (35 / 38) ;特异度是 96 .4 % (2 7/ 2 8)。结论　DNA序列分析对判断结核分支杆菌耐利福平非常有价值 ;PCR -SSCP可用于利福平耐药结核分支杆菌的初步筛选。

引用

页码：61 / 64

页数：4

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