粉红聚端孢菌胞外几丁质酶纯化、特性及抗菌活性

被引：10

作者：

张世宏

李多川

魏毅

刘开启

机构：

[1] 山东农业大学植保系

[2] 山东棉花研究中心

来源：

植物病理学报 | 2002年 / 03期

关键词：

粉红聚端孢菌; 几丁质酶; 纯化; 特性; 抗菌;

D O I：

10.13926/j.cnki.apps.2002.03.012

中图分类号：

S476 [生物防治];

学科分类号：

摘要：

粉红聚端孢菌 (Trichothecium roseum)在 SMCS中恒温摇床 (2 0 0 r/ min,2 8℃ )上培养 12 d,诱导产生了大量的胞外几丁质酶。培养滤液经 (NH4) 2 SO4分级沉淀、DEAE- Sepharose FF阴离子交换柱层析和 CM- Sepharose FF阳离子交换柱层析获得了 SDS- PAGE纯的胞外几丁质酶 ,分子量约为39k Da。几丁质酶最适作用温度为 4 0℃ ,最适 p H范围为 4 .0～ 7.0。抗菌活性显示 ,纯酶对供试病原菌都有不同程度的抑菌作用 ,其中对棉花黄萎菌的抑制作用最强。

引用

页码：262 / 266

页数：5

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