白血病细胞核因子-κB活化与化疗药物诱导凋亡的关系

被引:6
作者
许小平
史剑慧
李林
张宗梁
程文英
机构
[1] 第二军医大学长海医院血液科,复旦大学放射医学研究所,复旦大学放射医学研究所,中国科学院上海细胞生物研究所分子细胞生物学开放实验室,复旦大学放射医学研究所上海
关键词
白血病细胞; P388; 抗肿瘤药; 细胞凋亡; 细胞核因子-κB;
D O I
暂无
中图分类号
R733.7 [白血病];
学科分类号
100214 ;
摘要
目的 研究化疗药物诱导P388白血病细胞核因子 κB (NF κB)活化与凋亡的关系 ,以及长春新碱 (VCR)对它们的影响。方法 采用电泳迁移率变动分析 (EMSA)检测细胞NF κB活化水平 ;采用TdT介导的dUTP缺口末端标记技术 (TUNEL)和DNA电泳方法检测细胞凋亡。结果 化疗药物诱导P388白血病细胞NF κB活化与化疗药物诱导细胞凋亡有明显关系 ,0 .1μmol LVCR不仅能显著抑制 10 0 μmol L阿糖胞苷 (Ara C)或 10 0 μmol L鬼臼乙叉甙 (Vp 16 )诱导的P388细胞NF κB活化 (抑制率分别为 5 2 %和 6 3% ) ,而且增强它们诱导P388细胞凋亡的作用 (凋亡增加率分别为 89%和 12 3% )。P388细胞在接触化疗药物前 ,NF κB亦有一定程度的活化。结论 化疗药物诱导P388白血病细胞凋亡的同时 ,可活化NF κB ;VCR可通过抑制NF κB活化 ,增强化疗药物诱导白血病细胞凋亡的作用。
引用
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页码:12 / 15
页数:4
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