石蜡包埋尤文瘤/外周原始神经外胚瘤中EWS-FLI1融合基因的表达

被引：9

作者：

李锋

常彬

李新霞

庞丽娟

陆红芬

王坚

孙孟红

施达仁

机构：

[1] 石河子大学新疆地方与民族高发病省部共建教育部重点实验室医学院病理学教研室,石河子大学新疆地方与民族高发病省部共建教育部重点实验室医学院病理学教研室,石河子大学新疆地方与民族高发病省部共建教育部重点实验室医学院病理学教研室,石河子大学新疆地方与民族高发病省部共建教育部重点实验室医学院病理学教研室,复旦大学肿瘤医院病理科,复旦大学肿瘤医院病理科,复旦大学肿瘤医院病理科,复旦大学肿瘤医院病理科,,,

来源：

中华病理学杂志 | 2004年 / 04期

关键词：

肉瘤,Ewing; 神经外胚瘤,原始,外周; 基因表达; 石蜡包埋; 逆转录聚合酶链反应;

D O I：

暂无

中图分类号：

R730.4 [肿瘤诊断学];

学科分类号：

100214 ;

摘要：

目的运用逆转录聚合酶链反应(RT-PCR)技术检测石蜡包埋尤文瘤/外周原始神经外胚瘤(ES/pPNET)组织中特异性染色体易位融合基因EWS-FLIl/ERG mRNA的表达并探讨其诊断意义。方法运用一步法RT-PCR技术检测25例石蜡包埋ES/pPNETS和15例其他小圆细胞肿瘤(包括8例胚胎型和腺泡型横纹肌肉瘤、4例低分化滑膜肉瘤、2例神经母细胞瘤和1例淋巴瘤)组织中EWS-FLIl/ERG的表达。结果25例ES/pPNET中20例检测到EWS-FLIl/ERG融合基因的表达(80％),15例对照组均未检出EWS-FLIl的表达。结论 EWS-FLIl/ERG mRNA的表达是ES/pPNETs分子诊断的可靠指标,一步法RT-PCR是一种适用于临床常规石蜡包埋组织EWS-FLIl融合基因表达的检测方法。

引用

页码：30 / 33

页数：4

共 11 条

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