橡胶树冷应答转录组cDNA-AFLP分析

被引：27

作者：

安泽伟 ^{[1
,2
]}

陈根辉 ^{[3
]}

程汉 ^{[1
]}

赵彦宏 ^{[4
]}

谢黎黎 ^{[1
,2
]}

黄华孙 ^{[1
]}

机构：

[1] 中国热带农业科学院橡胶研究所国家橡胶树育种中心

[2] 海南大学农学院

[3] 福建省龙岩市农业科学研究所

[4] 鲁东大学生命科学学院

来源：

林业科学 | 2010年 / 46卷 / 03期

关键词：

橡胶树; cDNA-AFLP; 胁迫; 抗寒性; 分子育种;

D O I：

暂无

中图分类号：

S794.1 [橡胶树];

学科分类号：

0829 ; 0907 ;

摘要：

以橡胶树11个抗寒无性系和12个不抗寒无性系为研究对象,利用cDNA-AFLP技术对23个橡胶树无性系低温处理前后的基因表达谱进行差异分析。结果表明:通过100对引物组合的扩增筛选,共获得12条在2组材料中表现一致的差异片段(GenBank登录号:GO269543-GO269554);BLASTn和BLASTx比对分析显示,TDF7-8,TDF5-15,TDF16-103条片段分别与植物抗逆相关的ABC转运蛋白、3-脱氧-D-阿拉伯庚酮糖-7-磷酸合成酶(DAHPS)、WRKY基因有较高相似性,其余9条片段在GenBank未比对到同源序列。

引用

页码：62 / 67

页数：6

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