骨髓间充质干细胞诱导肝纤维化大鼠肝星状细胞凋亡和Caspase-3表达

被引：7

作者：

梁梓宇

覃山羽

姜海行

王东旭

苏思标

陈传华

机构：

[1] 广西医科大学第一附属医院消化内科

来源：

重庆医学 | 2010年 / 39卷 / 20期

关键词：

骨髓间充质干细胞; 肝纤维化大鼠; 肝星状细胞; 凋亡; Caspase-3;

D O I：

暂无

中图分类号：

R575.2 [肝硬变];

学科分类号：

1002 ; 100201 ;

摘要：

目的研究大鼠骨髓间充质干细胞(MSCs)诱导肝纤维化大鼠肝星状细胞(HSCs)凋亡及Caspase-3表达。方法分离培养大鼠MSCs。将肝纤维化模型SD大鼠60只平均分为A(鼠尾静脉注射等量的生理盐水)、B(鼠尾静脉注射含MSCs细胞悬液)、C(鼠尾静脉注射经肝细胞生长因子诱导14 d后的MSCs细胞悬液)组。鼠尾静脉输注2×105MSCs细胞悬液,于第1、2、3、4周末取肝组织,经HE、Masson染色观察肝纤维化程度,用TUNEL法检测各组HSCs凋亡,免疫组化检测Desmin,RT-PCR和Western Blot检测凋亡因子Caspase-3。结果 B、C两组肝组织学改善明显,肝纤维化程度减轻,HSCs数量明显减少,Desmin、TUNEL染色阳性的细胞均增多,Caspase-3基因mRNA和蛋白表达增强,且呈时间依赖性,与A组比较差异有统计学意义,且C组较B组明显(P<0.01,P<0.05)。结论 MSCs可在体内诱导HSCs凋亡并上调Caspase-3表达,肝细胞生长因子诱导MSCs抗肝纤维化较单纯MSCs作用更强。

引用

页码：2721 / 2723

页数：3

共 5 条

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