聚乙二醇对猪凝血酶的化学修饰及其效果分析

被引:3
作者
周建刚
谭远友
机构
[1] 武汉科技学院环境与城建学院
关键词
凝血酶; 聚乙二醇; 亲和层析;
D O I
10.14088/j.cnki.issn0439-8114.2009.06.002
中图分类号
Q814 [酶工程];
学科分类号
082203 ;
摘要
利用亲和层析法,从粗酶中纯化得到猪凝血酶。采用活化的单甲氧基聚乙二醇对凝血酶进行了共价修饰,以此修饰酶为研究对象,进行了凝血酶在修饰前后的主要酶学性质的比较研究。结果表明,凝血酶经mPEG1修饰后,Km有所增加,pH和盐离子浓度的稳定性、热稳定性以及抗胰蛋白酶的水解能力均有所增强。
引用
收藏
页码:1432 / 1435
页数:4
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