以绿色荧光蛋白标记干酪乳杆菌的构建

被引：3

作者：

丁轲 ^{[1
]}

丁盼盼 ^{[1
]}

余祖华 ^{[2
]}

李旺 ^{[1
]}

李元晓 ^{[1
]}

曹平华 ^{[1
]}

程相朝 ^{[2
,3
]}

张春杰 ^{[2
,3
]}

机构：

[1] 河南科技大学宏翔生物饲料实验室

[2] 河南省动物疫病与公共安全院士工作站

[3] 河南省高等学校环境与畜产品安全重点学科开放实验室

来源：

黑龙江畜牧兽医 | 2014年 / 19期

关键词：

绿色荧光蛋白; 干酪乳杆菌; 构建; 稳定性; 电转化;

D O I：

10.13881/j.cnki.hljxmsy.2014.1101

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

为了更直接、更方便检测乳酸杆菌表达系统,研究通过PCR法以质粒pEGFP-C1为模板扩增出增强型绿色荧光蛋白基因(egfp)序列,将扩增产物连接到乳酸杆菌的整合型载体pMJ67上,成功构建了重组质粒pMJ-egfp。将质粒pMJ-egfp电转化入乳酸杆菌L.casei CECT5276,egfp编码序列通过质粒上的Lac操纵子同源重组整合到L.casei CECT5276染色体上,连续传代50次,发现绿色荧光蛋白能够稳定遗传。说明试验获得了能在无抗性选择压力下稳定表达绿色荧光蛋白的重组菌L.casei CECT5278。

引用

页码：22 / 24+28+227 +28

页数：5

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