基于AFLP和SSR标记的高粱分子遗传连锁图构建

被引：5

作者：

卞云龙 ^{[1
]}

邓德祥 ^{[1
]}

王益军 ^{[1
]}

才宏伟 ^{[2
]}

机构：

[1] 扬州大学农学院江苏省作物遗传生理重点实验室,扬州大学农学院植物功能基因组学教育部重点实验室

[2] 日本草地畜产种子协会饲料作物研究所

来源：

分子植物育种 | 2007年 / 05期

关键词：

高粱; 遗传图谱; AFLP; SSR;

D O I：

暂无

中图分类号：

S514 [高粱];

学科分类号：

0901 ;

摘要：

以茎秆糖份含量高的高粱自交系1095和低糖高粱自交系N3杂交获得的F2分离群体(205个个体)为材料,采用AFLP(amplified fragment length polymorphism)和SSR(simple sequence repeat)两种分子标记,构建了包含273个(232AFLP,41SSR)标记,覆盖基因组长度为978.1cM的高粱分子标记连锁遗传图。以SSR标记为锚标记,19个连锁群中,18个连锁群各自被归并于高粱的10个连锁群(A-J)中。该连锁图平均图距和最大图距分别为3.6cM和19.4cM,未出现大的空隙(gap>25cM),归并后的10个连锁群(A-J)分别对应于高粱染色体SBI-01、SBI-02、SBI-03、SBI-04、SBI-07、SBI-09、SBI-10、SBI-08、SBI-06、SBI-05。

引用

页码：661 / 666

页数：6

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