嗜水气单胞菌侵袭力与宿主细胞信号转导和骨架的关系

Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm r)bacteria from Triton X-100 cell lysates. The invasive efficiency (IE) could reach to 0.2% at 37℃. In addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. Bacterial internalization of Ah J-1 involved microfilaments and protein tyrosine kinase since cytochalasin D (an inhibitor of microfilament polymerization) and genistein (an inhibitor of protein tyrosine kinase) prevented internalization. Staurosporine(a protein kinase C inhibitor) and Sodium orthovanadate (a protein tyrosine phosphatase inhibitor) accelerated internalization of Ah J-1 into HEp-2 cells.