游泳运动对大鼠膝关节骨关节炎内质网应激PERK表达的影响

被引：5

作者：

谢冰 ^{[1
]}

肖乐 ^{[1
]}

刘超 ^{[1
]}

李元 ^{[1
]}

孔瑛 ^{[2
]}

机构：

[1] 长沙市第一医院康复医学科

[2] 中南大学湘雅二医院

来源：

中国康复 | 2018年 / 05期

关键词：

游泳运动; 骨关节炎; PERK; 内质网应激;

D O I：

暂无

中图分类号：

R684.3 [关节炎];

学科分类号：

100220 [骨科学];

摘要：

目的:通过研究游泳运动对内质网应激PERK表达的影响,探讨游泳运动治疗膝关节骨关节炎可能的作用机制。方法:将雄性健康SD大鼠24只随机分为空白对照组(A组)、假手术组(B组),模型对照组(C组)和游泳运动组(D组),每组6只。A组笼内自由活动8周,B组假手术后笼内自由活动8周,C组造模后笼内自由活动8周,D组造模后笼内自由活动4周后进行游泳训练4周。治疗结束后处死大鼠,观察关节内情况,并进行大体评分,行HE染色及甲苯胺蓝染色,进行改良Mankin评分,免疫组化法检测PERK的表达。结果:大体观察及评分、HE染色、甲苯胺蓝染色显示C、D组关节软骨损伤程度较A组严重,C、D组与A组评分比较明显增高(P<0.05);B组与A组比较差异无统计学意义;D组与C组比较评分降低(P<0.05)。PERK的表达C、D组较A、B组增高(P<0.05);D组较C组PERK阳性细胞数降低(P<0.05);B组与A组比较差异无统计学意义。结论:游泳运动可对膝关节骨关节炎起治疗作用,其作用机制之一可能是通过减少PERK的表达,降低内质网应激,减少软骨细胞凋亡实现的。

引用

页码：355 / 359

页数：5

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